Shouldn’t bother them, even though they are zero loss couplers same theory as the Pioneer quick couplers all out farm equipment uses, having pressure on em won’t hurt em.
If it’s the same as a skid steer I’d just hook one hose up and run it and see what happens, it should work as a closed system once it sense pressure on the circuit that’s not hooked to anything the pump should shut down as it’s reached the cut out pressure.
All our tractors are pressure compensating systems on the newer/bigger ones and we run single acting cylinders on all of em at one time or another.
If nothing else call the closest Bobcat dealer and ask em.