Oxy barrier for sure, .......radiant barrier?
What are you using? Hopefully you meant foam board. 2".... I would go 6" oc which is about 1600' of 1/2" pex.. 5/8" @ 9"oc is 1200' pex. Try to keep loops at 250' , longer than this creates inconsistencies or requires over pumping to hide. Also you want your mesh or pex on chairs so it's within 2" of the top of Crete. This reduces lag time greatly and also allows for fewer btu and lower supply temps.
Don't forget to insulate the edges as well this accounts for 15-27% of losses. I would do a standard layout not reverse return since this is a barn. Meaning the hottest goes to the perimeter and works toward the center. Don't be afraid of going shorter loops and more loops. It can't hurt, but too long can hurt. This gives you much more control and flexibility. Maybe you section off a room and want more heat there, just balance the manifold to do so, for example.